anti agrin (Novus Biologicals)
Structured Review
Anti Agrin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agrn/Agrin+Antibody/pm41887219-989-47-49
Average 94 stars, based on 7 article reviews
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SDS Page:Article Title: Cancer-cell-derived matrisome proteins promote metastasis in pancreatic ductal adenocarcinoma Article Snippet: Primary antibodies used were: AGRN (Novus Biologicals, Littleton, CO, NBP1–90209), CDH1 (BD Biosciences, San Jose, CA, 610181), CSTB (Abcam, Cambridge, MA, ab53725), LMNA (human specific; Abcam, ab108595), SERPINB5 (Origene, Rockville, MD, TA-322980), VIM (human specific; Leica Biosystems, Nussloch, Germany, NCL-L-VIM-V9), ZEB1 (Sigma-Aldrich, St. Louis, MO, HPA027524). .. Tumor samples were lysed in Laemmli buffer, proteins were separated by SDS-PAGE (4–20% gradient gel from Biorad) and immunoblotting was performed using the following antibodies: GAPDH (Millipore, Bedford, MA, MAB374), Article Title: Cancer-cell-derived matrisome proteins promote metastasis in pancreatic ductal adenocarcinoma Article Snippet: Primary antibodies used were: AGRN (Novus Biologicals, Littleton, CO, NBP1–90209), CDH1 (BD Biosciences, San Jose, CA, 610181), CSTB (Abcam, Cambridge, MA, ab53725), LMNA (human specific; Abcam, ab108595), SERPINB5 (Origene, Rockville, MD, TA-322980), VIM (human specific; Leica Biosystems, Nussloch, Germany, NCL-L-VIM-V9), ZEB1 (Sigma-Aldrich, St. Louis, MO, HPA027524). .. Immunoblotting of tumor samples, cells and cell culture media Tumor samples were lysed in Laemmli buffer, proteins were separated by SDS-PAGE (4–20% gradient gel from Biorad) and immunoblotting was performed using the following antibodies: GAPDH (Millipore, Bedford, MA, MAB374), Western Blot:Article Title: Cancer-cell-derived matrisome proteins promote metastasis in pancreatic ductal adenocarcinoma Article Snippet: Primary antibodies used were: AGRN (Novus Biologicals, Littleton, CO, NBP1–90209), CDH1 (BD Biosciences, San Jose, CA, 610181), CSTB (Abcam, Cambridge, MA, ab53725), LMNA (human specific; Abcam, ab108595), SERPINB5 (Origene, Rockville, MD, TA-322980), VIM (human specific; Leica Biosystems, Nussloch, Germany, NCL-L-VIM-V9), ZEB1 (Sigma-Aldrich, St. Louis, MO, HPA027524). .. Tumor samples were lysed in Laemmli buffer, proteins were separated by SDS-PAGE (4–20% gradient gel from Biorad) and immunoblotting was performed using the following antibodies: GAPDH (Millipore, Bedford, MA, MAB374), Article Title: Cancer-cell-derived matrisome proteins promote metastasis in pancreatic ductal adenocarcinoma Article Snippet: Primary antibodies used were: AGRN (Novus Biologicals, Littleton, CO, NBP1–90209), CDH1 (BD Biosciences, San Jose, CA, 610181), CSTB (Abcam, Cambridge, MA, ab53725), LMNA (human specific; Abcam, ab108595), SERPINB5 (Origene, Rockville, MD, TA-322980), VIM (human specific; Leica Biosystems, Nussloch, Germany, NCL-L-VIM-V9), ZEB1 (Sigma-Aldrich, St. Louis, MO, HPA027524). .. Immunoblotting of tumor samples, cells and cell culture media Tumor samples were lysed in Laemmli buffer, proteins were separated by SDS-PAGE (4–20% gradient gel from Biorad) and immunoblotting was performed using the following antibodies: GAPDH (Millipore, Bedford, MA, MAB374), Cell Culture:Article Title: Cancer-cell-derived matrisome proteins promote metastasis in pancreatic ductal adenocarcinoma Article Snippet: Primary antibodies used were: AGRN (Novus Biologicals, Littleton, CO, NBP1–90209), CDH1 (BD Biosciences, San Jose, CA, 610181), CSTB (Abcam, Cambridge, MA, ab53725), LMNA (human specific; Abcam, ab108595), SERPINB5 (Origene, Rockville, MD, TA-322980), VIM (human specific; Leica Biosystems, Nussloch, Germany, NCL-L-VIM-V9), ZEB1 (Sigma-Aldrich, St. Louis, MO, HPA027524). .. Immunoblotting of tumor samples, cells and cell culture media Tumor samples were lysed in Laemmli buffer, proteins were separated by SDS-PAGE (4–20% gradient gel from Biorad) and immunoblotting was performed using the following antibodies: GAPDH (Millipore, Bedford, MA, MAB374), other:Article Title: Cancer-cell-derived matrisome proteins promote metastasis in pancreatic ductal adenocarcinoma Article Snippet: Both control and experimental mice were fed with DOX diet (Bio-serv) throughout the experiment. Article Title: Cancer-cell-derived matrisome proteins promote metastasis in pancreatic ductal adenocarcinoma Article Snippet: Orthotopic and tail-vein implantation For CRISPR-SAM-activation and CRISPR-inactivation experiments, 1×10 5 cells in 50ul PBS were injected into pancreata of 8–10-week-old NOD/SCID/IL2Rγ-null (NSG) mice (Jackson Laboratory, Bar Harbor, ME). Article Title: Cancer-cell-derived matrisome proteins promote metastasis in pancreatic ductal adenocarcinoma Article Snippet: BxPC3 G1.1 cells were transduced with pHR-TRE3G-KRAB-dCas9-P2A-Blast lentivirus, which is modified from a construct (Addgene #60954) by replacing SFFV promoter with a doxycyclin-inducible promoter TRE3G and mCherry with Blasticidin sequence, and selected with Blasticidin (16). |
